李健课题组介绍
课题组长 | ![]() |
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研究兴趣 | ![]() |
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组内动态 | ![]() |
2022.3 祝贺田昕彤在Synthetic and Systems Biotechnology上发表研究论文! 2022.3 祝贺刘雨诗在ACS Catalysis上发表研究论文! 新闻链接 2022.3 李健受邀担任Frontiers in Natural Products杂志创刊副主编 (Associate Editor),负责天然产物生物合成方向稿件。 2022.2 祝贺季向阳和刘晚秋以共同第一作者在Current Opinion in Microbiology上发表综述论文! 2022.2 祝贺巴方在Nucleic Acids Research上发表研究论文! 学校新闻网以“合成生物学新技术给微生物穿上七彩‘服饰’”为题进行了报道。新闻链接 2022.2 祝贺许慧玲在ACS Synthetic Biology上发表研究论文! 2021.9 祝贺杨晨通过硕士论文答辩! 2021.9 欢迎卢屹聪和张雨菲同学加入课题组! 2021.9 李健受邀继续担任Frontiers in Bioengineering and Biotechnology 客座编辑(Guest Topic Editor)出版以“Cell-Free Synthetic Biology, Volume II”为主题的第二期研究专刊(Research Topic)。https://www.frontiersin.org/research-topics/26520/cell-free-synthetic-biology-volume-ii 2021.8 祝贺刘晚秋获得国家自然科学基金面上项目资助! 2021.7 祝贺杨晨在Frontiers in Bioengineering and Biotechnology上发表研究论文! 2021.7 实验室组织多人参加中国生物工程学会第五届青年科技论坛,李健做题为《无细胞合成生物学驱动高效生物合成》的会议报告。 2021.6 祝贺巴方通过转博为博士研究生! 2021.5 实验室组织多人参加第四届生物化工青年学者论坛,大家通过参会广泛了解研究进展,并与学术同行进行了深入交流,扩展了大家的视野。 2021.4 实验室组织前往上海迪士尼春游活动,学习之余大家放松了身心。 2021.4 祝贺黄曙惠和刘雨诗以共同第一作者在Microorganisms上发表关于缬氨霉素 (Valinomycin) 的综述论文! 2021.3 李健参加德国第六届BioProScale在线会议并做Poster报告,同时获得会议Poster奖 (2nd Place Award)! 2021.3 课题组邀请清华大学卢元副教授到实验室交流,并做题为《无细胞合成生物学:从原型设计到应用拓展》的学术报告。 2021.2 祝贺杨晨以共同第一作者在ACS Synthetic Biology上发表研究论文! 2021.1 课题组举行2020年年终总结交流会,大家总结了过去,展望了未来,对新的一年充满了憧憬! 2020.12 实验室组织多人参加2020年上海市微生物学会/生物工程学会青年论坛,李健受邀做会议报告,许慧玲获得论坛“优秀墙报”奖! 2020.10 实验室组织农家乐秋游活动,挖红薯、垂钓、烧烤、聚餐...大家通过这次活动放松了身心,增进了交流! 2020.9 欢迎郑霄、孟雅如、刘颖、陈伊琳和卢皓凡同学加入课题组! 2020.8 祝贺刘晚秋在Biotechnology and Bioengineering上发表研究论文! 2020.7 祝贺黄曙惠和许慧玲通过转博为博士研究生! 2020.5 欢迎巴方同学加入课题组! 2020.5 学校新闻网报道课题组在无细胞蛋白合成新体系研究上取得系列进展!新闻链接 2020.5 祝贺张凌凯在Frontiers in Bioengineering and Biotechnology上发表研究论文! 2020.4 祝贺许慧玲在ACS Synthetic Biology上发表研究论文! 2020.4 学校新闻网报道课题组利用无细胞合成生物学在复杂天然产物全合成方面取得进展!新闻链接 2020.3 祝贺庄蕾和黄曙惠以共同第一作者在Metabolic Engineering上发表研究论文! 2019.12 课题组邀请上海交通大学林双君教授到实验室交流,并做题为《微生物生物碱生物合成》的学术报告。 2019.11 课题组邀请中科院上海生命科学研究院植物生理生态研究所王勇研究员到实验室交流,并做题为Natural Product Synthetic Biology: From Bacteria to Plant的学术报告。 2019.10 李健受邀担任Frontiers in Bioengineering and Biotechnology 客座编辑(Guest Topic Editor)出版以“Cell-Free Synthetic Biology”为主题的研究专刊(Research Topic)。https://www.frontiersin.org/research-topics/12103/cell-free-synthetic-biology 2019.9 课题组邀请复旦大学张琪教授到实验室交流,并做题为Discovery and Biosynthesis of Ribosomally Synthesized and Posttranslationally Modified Peptides (RiPPs)的学术报告。
2019.9 欢迎田昕彤、季向阳和张任三位同学加入课题组! 2019.8 李健获得国家自然科学基金面上项目资助! 2019.7 欢迎刘雨诗博士加入课题组,进行博士后研究! 2019.7 欢迎阿根廷布宜诺斯艾利斯大学(University of Buenos Aires)的Julieta Viglino同学加入课题组开展暑期科研项目! 2019.6 祝贺2019届毕业生庄蕾获得上海科技大学优秀毕业生、上海市优秀毕业生和上海科技大学本科毕业生校长奖!同时,庄蕾将前往美国罗格斯大学(Rutgers, The State University of New Jersey)攻读博士学位,祝她一切顺利! 2019.6 欢迎美国芝加哥大学(The University of Chicago)的Alexis Vitiello同学加入课题组开展暑期科研项目! 2019.5 李健获得上海市自然科学基金资助! 2019.4 李健参加中国生物工程学会第四届青年科技论坛并做大会报告,会前,李健入选为中国生物工程学会青年工作委员会委员。 2019.4 实验室组织前往上海野生动物园一日游,科研之余,大家放松了心情,加强了交流,增进了感情! 2019.3 李健受邀参加由日本生物工学会主办的首届Sakura-Bio会议并做大会报告。 2018.11 课题组现诚聘博士后1名,要求:具有或即将获得生物工程、微生物学、生物化学与分子生物学、合成生物学、生物化工、化学等相关专业博士学位。有意者请与李健邮件联系。 2018.10 李健担任共同指导老师带领上海科技大学iGEM团队获得2018年国际基因工程机器大赛(International Genetically Engineered Machine Competition, iGEM)全球总决赛金牌。 2018.9 欢迎黄曙惠、杨晨和许慧玲三位同学加入课题组! 2018.8 祝贺刘晚秋获得国家自然科学基金青年项目资助! 2018.8 李健受邀担任Springer Nature出版社《SN Applied Sciences》杂志编委。 2018.7 李健参加第四届合成生物学青年学者论坛(Synthetic Biology Young Scholar Forum)并做大会报告。 2018.1 欢迎刘晚秋博士加入课题组,任助理研究员! 2017.9 欢迎张凌凯同学加入课题组! 2017.9 上海科技大学无细胞合成生物学(Cell-Free Synthetic Biology)课题组正式成立! |
发表论文 | ![]() |
(*Corresponding author, #Equal contribution)
32. Yang C#, Yang M#, Zhao W, Ding Y, Wang Y*, Li J*. Establishing a Klebsiella pneumoniae-based cell-free protein synthesis system. Molecules, 2022, 27, 4684 31. Sun Z, Hübner R, Li J, Wu C. Artificially sporulated Escherichia coli cells as a robust cell factory for interfacial biocatalysis. Nature Communications, 2022, 13, 3142 30. Zhang Y, Chen W, Wu D, Liu Y, Wu Z, Li J, Zhang SY, Ji Q. Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli. Nucleic Acids Research, 2022, 50, 5948–5960 29. Li J*. Engineered microbes: Making valuable chemicals from waste gases. Synthetic and Systems Biotechnology, 2022, 7, 837–838 28. Tian X, Liu WQ, Xu H, Ji X, Liu Y, Li J*. Cell-free expression of NO synthase and P450 enzyme for the biosynthesis of an unnatural amino acid L-4-nitrotryptophan. Synthetic and Systems Biotechnology, 2022, 7, 775–783 27. Liu Y, Ba F, Liu WQ, Wu C, Li J*. Plug-and-play functionalization of protein-polymer conjugates for tunable catalysis enabled by genetically encoded "click" chemistry. ACS Catalysis, 2022, 12, 4165–4174 26. Ji X#, Liu WQ#, Li J*. Recent advances in applying cell-free systems for high-value and complex natural product biosynthesis. Current Opinion in Microbiology, 2022, 67, 102142 (Invited review) 25. Ba F, Liu Y, Liu WQ, Tian X, Li J*. SYMBIOSIS: Synthetic manipulable biobricks via orthogonal serine integrase systems. Nucleic Acids Research, 2022, 50, 2973–2985 24. Xu H, Yang C, Tian X, Chen Y, Liu WQ, Li J*. Regulatory part engineering for high-yield protein synthesis in an all-Streptomyces-based cell-free expression system. ACS Synthetic Biology, 2022, 11, 570–578 23. Xu H, Liu WQ, Li J*. A Streptomyces-based cell-free protein synthesis system for high-level protein expression. Methods in Molecular Biology, 2022, 2433, 89–103 22. Li J*, Kwon YC*, Lu Y*, Moore SJ*. Editorial: Cell-free synthetic biology. Frontiers in Bioengineering and Biotechnology, 2021, 9, 799122
21. Yang C, Liu Y, Liu WQ, Wu C, Li J*. Designing modular cell-free systems for tunable biotransformation of L-phenylalanine to aromatic compounds. Frontiers in Bioengineering and Biotechnology, 2021, 9, 730663 20. Huang S#, Liu Y#, Liu WQ, Neubauer P*, Li J*. The nonribosomal peptide valinomycin: From discovery to bioactivity and biosynthesis. Microorganisms, 2021, 9, 780 19. Feng J#, Yang C#, Zhao Z, Xu J, Li J*, Li P*. Application of cell-free protein synthesis system for the biosynthesis of L-theanine. ACS Synthetic Biology, 2021, 10, 620–631 18. Liu WQ, Wu C*, Jewett MC, Li J*. Cell-free protein synthesis enables one-pot cascade biotransformation in an aqueous-organic biphasic system. Biotechnology and Bioengineering, 2020, 117, 4001–4008 17. Zhang L, Liu WQ, Li J*. Establishing a eukaryotic Pichia pastoris cell-free protein synthesis system. Frontiers in Bioengineering and Biotechnology, 2020, 8, 536 16. Xu H, Liu WQ, Li J*. Translation related factors improve the productivity of a Streptomyces-based cell-free protein synthesis system. ACS Synthetic Biology, 2020, 9, 1221–1224 15. Zhuang L#, Huang S#, Liu WQ, Karim AS, Jewett MC*, Li J*. Total in vitro biosynthesis of the nonribosomal macrolactone peptide valinomycin. Metabolic Engineering, 2020, 60, 37–44 14. Liu WQ#, Zhang L#, Chen M#, Li J*. Cell-free protein synthesis: Recent advances in bacterial extract sources and expanded applications. Biochemical Engineering Journal, 2019, 141, 182–189 13. Li J*, Zhang L, Liu WQ. Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products. Synthetic and Systems Biotechnology, 2018, 3, 83–89 12.Wang H, Li J*, Jewett MC*. Development of a Pseudomonas putida cell-free protein synthesis platform for rapid screening of gene regulatory elements. Synthetic Biology, 2018, 3, ysy003 PhD and Postdoc publications 11.Casini A, Chang FY, Eluere R, King A, Young EM, Dudley QM, Karim A, Pratt K, Bristol C, Forget A, Ghodasara A, Warden-Rothman R, Gan R, Cristofaro A, Espah Borujeni A, Ryu MH, Li J, Kwon YC, Wang H, Tatsis E, Rodriguez-Lopez C, O'Connor S, Medema MH, Fischbach M, Jewett MC, Voigt CA, Gordon DB. A pressure test to make 10 molecules in 90 days: external evaluation of methods to engineer biology. Journal of the American Chemical Society, 2018, 140, 4302–4316 10. Li J, Wang H, Jewett MC. Expanding the palette of Streptomyces-based cell-free protein synthesis systems with enhanced yields. Biochemical Engineering Journal, 2018, 130, 29–33 9. Li J, Wang H, Kwon YC, Jewett MC. Establishing a high yielding Streptomyces-based cell-free protein synthesis system. Biotechnology and Bioengineering, 2017, 114, 1343–1353 (Video Highlighted) 8. Goering AW#, Li J#, McClure RA, Thomson RJ, Jewett MC, Kelleher NL. In vitro reconstruction of nonribosomal peptide biosynthesis directly from DNA using cell-free protein synthesis. ACS Synthetic Biology, 2017, 6, 39–44 7. Li J, Lawton TJ, Kostecki JS, Nisthal A, Fang J, Mayo SL, Rosenzweig AC, Jewett MC. Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase. Biotechnology Journal, 2016, 11, 212–218 (Front Cover) 6. Moatsou D#, Li J#, Ranji A, Pitto-Barry A, Ntai I, Jewett MC, O’Reilly RK. Self-assembly of temperature-responsive protein-polymer bioconjugates. Bioconjugate Chemistry, 2015, 26, 1890–1899 (Front Cover) 5. Li J*, Jaitzig J, Lu P, Süssmuth RD, Neubauer P. Scale-up bioprocess development for production of the antibiotic valinomycin in Escherichia coli based on consistent fed-batch cultivations. Microbial Cell Factories, 2015, 14, 83 4. Li J*, Jaitzig J, Theuer L, Legala OE, Süssmuth RD, Neubauer P. Type II thioesterase improves heterologous biosynthesis of valinomycin in Escherichia coli. Journal of Biotechnology, 2015, 193, 16–22 3. Li J*, Neubauer P. Escherichia coli as a cell factory for heterologous production of nonribosomal peptides and polyketides. New Biotechnology, 2014, 31, 579–585 2. Li J#, Jaitzig J#, Hillig F, Süssmuth RD, Neubauer P. Enhanced production of the nonribosomal peptide antibiotic valinomycin in Escherichia coli through small-scale high cell density fed-batch cultivation. Applied Microbiology and Biotechnology, 2014, 98, 591–601 1. Jaitzig J#, Li J#, Süssmuth RD, Neubauer P. Reconstituted biosynthesis of the nonribosomal macrolactone antibiotic valinomycin in Escherichia coli. ACS Synthetic Biology, 2014, 3, 432–438 |
课题组照片 | ![]() |
2021年10月
2020年10月
2019年10月
2018年10月
本组成员 | ![]() |
刘晚秋助理研究员复旦大学 博士后中科院微生物所 博士
| 刘雨诗博士后西北农林科技大学 博士 | ||
黄曙惠博士研究生
| 许慧玲博士研究生
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巴方博士研究生
| 田昕彤硕士研究生
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季向阳硕士研究生
| 张任硕士研究生
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郑霄硕士研究生
| 孟雅如硕士研究生
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刘颖硕士研究生
| 陈伊琳硕士研究生
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卢皓凡硕士研究生 | 卢屹聪硕士研究生 | ||
张雨菲硕士研究生 |
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Alumni | ![]() |
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